Centella asiatica is considered as
an important medicinal plant enriched with several bioactive compounds like
triterpenoid Saponins that includes asiaticoside, madecassoside, and
centelloside. This medicinal plant has playes a vital role in treatingbacterial, viral and parasitic infections such as urinary tract infections,leprosy, cholera, dysentery and common cold. Women in Srilanka use this
medicinal plant to prevent unwanted pregnancy and issues related to menstrual
cycle.
Shoot multiplication and
phytocompounds production from different accessions is required to be assessed
to choose an accession giving optimum production of phytocompounds. For this,different media containing different combinations of macro and micro nutrientshave to be tested that influence the growth of the plant. Assessment of best
culture media and concentrations of plant hormone for shoot culture are some of
the critical culture conditions to achieve. So there is a need to optimise such
conditions that will enhance the growth of the plant. For shoot culture,
explant of different accession of Centella asiatica were inoculated in
different media i.e. Murashige and Skoog (MS), Gamborg’s B5 and Nitsch medium
which were supplemented with standard concentrations of plant growth hormones.